歡迎您訪問武漢艾美捷科技有限公司官方網站!
                站點地圖 服務熱線:400-6800-868
                • 產品
                • 文章

                AGENT BRAND

                品牌專題

                當前位置:首頁 > 品牌專題

                Cell Biolabss:氧自由基抗氧化能力(ORAC)活性檢測試劑盒

                發布者:艾美捷科技    發布時間:2021-10-28     
                分享到:

                自由基與多種人類疾病的發生發展都有著密切的關系。在清除自由基的過程中,抗氧化劑發揮著重要的作用。因此,了解抗氧化劑的作用及能力就變得至關重要。

                 

                在評價抗氧化劑能力的諸多方法中,抗氧化能力ORAC(又稱,氧自由基吸收能力,Oxygen Radical Absorbance Capacity)分析法,被認定為檢測抗氧化劑抗氧化能力的標準方法,是目前普遍被接受的一個標準評價手段。由于該法具有接近機體生理條件、操作簡單、認可度和靈敏度高、準確性和重現性佳、高通量、不易受人為因素干擾等優點,被廣泛用于農業,食品,保健品,藥品及醫學領域。

                 

                自由基.jpg

                 

                那么如何分析樣本中氧自由基抗氧化能力/氧自由基吸收能力(ORAC)?作為專業的生命科學醫藥原料供應商,艾美捷科技為您推薦:眾多文章引用的,氧自由基抗氧化能力(ORAC)活性檢測試劑盒

                產品氧自由基抗氧化能力(ORAC)檢測試劑盒
                OxiSelect ORAC Activity Assay Kit
                貨號CBL-STA-345
                檢測方法熒光法
                適用樣本類型血清/血漿,尿液,細胞裂解液/提取物,組織裂解物,營養樣品(食品,植物等等)
                說明書下載點擊下載 請于實驗前,詳細閱讀說明書
                試劑盒原理ORAC分析法中的自由基主要來源于偶氮化合物2,2'-偶氮-雙-(2-瞇基丙烷)氯化二氫(2,2'-azobis(2-amidinopropane)dihydrochloride,AAPH)熱分解產生的過氧化氫自由基,也可以是芬頓(Fenton)反應過程中產生的羥自由基,以熒光素鈉(Sodium Flourescein,FL)為熒光探針,觀察自由基與熒光探針作用后,探針熒光強度的衰退過程,以水溶性維生素E類似物(Trolox)作為抗氧化標準物質,檢測體系中各種抗氧化劑延緩探針熒光強度衰退的能力,以此評價抗氧化劑的抗氧化能力。
                實驗優勢1 簡單快捷:不到2小時完成實驗;
                2 樣本適用:多樣性
                3 提供標準品;
                試劑盒組分1. 96-well Microtiter Plate
                2. Fluorescein Probe (100X)
                3. Free Radical Initiator
                4. Antioxidant Standard (Trolox)
                5. Assay Diluent (4X)
                保存條件收到后,Fluorescent Probe和Antioxidant Standard (Trolox) 保存于-20℃;其它所有試劑組分保存在4℃。

                * 本產品僅適用于科研用途.

                 

                氧自由基抗氧化能力(ORAC)活性檢測試劑盒,原理與標曲展示:

                 

                氧自由基抗氧化能力(ORAC)活性檢測試劑盒-原理示意圖.jpg

                氧自由基抗氧化能力(ORAC)活性檢測試劑盒-原理示意圖

                 

                氧自由基抗氧化能力(ORAC)活性檢測試劑盒-標曲示意圖.jpg

                氧自由基抗氧化能力(ORAC)活性檢測試劑盒-標曲示意圖

                 

                FAQ:

                 

                1.是否可以測試冷凍保存的樣品?

                RE:通常適用于在 -80℃ 下保存兩個月以內樣品,但我們建議盡可能測試新鮮樣品以獲得最佳結果

                 

                2.ORAC、HORAC和TAC檢測之間的區別,如何選擇?

                RE:所有三種測定都測量抗氧化能力,但略有不同。ORAC檢測分析氧自由基的抗氧化能力;HORAC檢測分析羥基自由基的抗氧化能力;TAC基于單電子轉移(SET)機制和銅離子,檢測總體抗氧化能力??傮w而言,研究人員更偏好選擇ORAC分析而不是HORAC,因為過氧自由基似乎比羥基自由基更相關。

                 

                3.更多常見問題,請點擊:cellbiolabs.com/faq/oxidative-stress-faq/orac-assay

                 

                近期發表文章(僅摘取部分展示):

                Rolnik, A. et al. (2021). Antioxidant and hemostatic properties of preparations from Asteraceae family and their chemical composition - Comparative studies. Biomed Pharmacother. 142:111982. doi: 10.1016/j.biopha.2021.111982.

                Xiao, L. et al. (2021). Hydrogen-Generating Silica Material Prevents UVA-ray-Induced Cellular Oxidative Stress, Cell Death, Collagen Loss and Melanogenesis in Human Cells and 3D Skin Equivalents. Antioxidants (Basel). 10(1):E76. doi: 10.3390/antiox10010076.

                Yuasa, M. et al. (2020). Antioxidant Activities and Taste Qualities of Fresh Onions Produced in Minamishimabara City, Nagasaki, Japan. J Food Sci Technol. doi: 10.3136/fstr.26.167.

                Gómez-Zorita, S. et al. (2020). Comparative Effects of Pterostilbene and Its Parent Compound Resveratrol on Oxidative Stress and Inflammation in Steatohepatitis Induced by High-Fat High-Fructose Feeding. Antioxidants (Basel). 9(11):E1042. doi: 10.3390/antiox9111042.

                Yuasa, M. et al. (2020). Antioxidant and taste properties of fresh onion (Allium cepa L.) leaves. J. Food Meas. Charact. doi: 10.1007/s11694-020-00704-w.

                Wake, H. et al. (2020). Histidine-rich glycoprotein possesses anti-oxidant activity through self-oxidation and inhibition of hydroxyl radical production via chelating divalent metal ions in Fenton's reaction. Free Radic Res. doi: 10.1080/10715762.2020.1825703.

                Suruga, K. et al. (2020). Soybean fermentation with basidiomycetes (medicinal mushroom mycelia). Chem. Biol. Technol. Agric. 7:23. doi: 10.1186/s40538-020-00189-1.

                Cheng, H.S. et al. (2020). Pleiotrosepic ameliorative effects of ellagitannin geraniin against metabolic syndrome induced by high-fat diet in rats. Nutrition. doi: 10.1016/j.nut.2020.110973.

                Jeon, S.Y. et al. (2020). Combined Extract of Vitis vinifera L. and Centella asiatica Synergistically Attenuates Oxidative Damage Induced by Hydrogen Peroxide in Human Umbilical Vein Endothelial Cells. Prev Nutr Food Sci. 25(2):173-183. doi: 10.3746/pnf.2020.25.2.173.

                Xiao, L. et al. (2020). Enzyme-digested Colla Corii Asini (E’jiao) prevents hydrogen peroxide-induced cell death and accelerates amyloid beta clearance in neuronal-like PC12 cells. Neural Regen Res. 15:2270-2. doi: 10.4103/1673-5374.285000.

                Yang, Y. et al. (2020). Chemical and cytological evaluation of honeybee pollen antioxidant ability. J Food Sci. doi: 10.1111/1750-3841.15047.

                Hu, R. et al. (2020). Antioxidant Performances of Corn Gluten Meal and DDGS Protein Hydrolysates in Food, Pet Food, and Feed Systems. Journal of Agriculture and Food Research. doi: 10.1016/j.jafr.2020.100030.

                Liu, Y.H. et al. (2020).  Acetylcholinesterase inhibitory activity and neuroprotection in vitro, molecular docking, and improved learning and memory functions of demethylcurcumin in scopolamine-induced amnesia ICR mice. Food Funct. doi: 10.1039/C9FO02339A.

                Kim, D. et al. (2020). Pig Skin Gelatin Hydrolysates Attenuate Acetylcholine Esterase Activity and Scopolamine-induced Impairment of Memory and Learning Ability of Mice. Food Sci Anim Resour. doi: 10.5851/kosfa.2020.e3.

                Mishima, E. et al. (2019). Drugs Repurposed as Antiferroptosis Agents Suppress Organ Damage, Including AKI, by Functioning as Lipid Peroxyl Radical Scavengers. J Am Soc Nephrol. pii: ASN.2019060570. doi: 10.1681/ASN.2019060570.

                Riquelme, S. et al. (2019). Bench-scale extraction of stilbenoids and other phenolics from stored grape canes (Vitis Vinifera):  Optimization process, chemical characterization, and potential protection against oxidative damage.  Journal of the Chilean Chemical Society. 64(2).

                Liu, Y.H. et al. (2019). Anti-glycation, anti-hemolysis, and ORAC activities of demethylcurcumin and tetrahydroxycurcumin in vitro and reductions of oxidative stress in D-galactose-induced BALB/c mice in vivo. Bot Stud. 60(1):9. doi: 10.1186/s40529-019-0258-x.

                Engstr m- st, J. et al. (2019). Eco-physiological responses of copepods and pteropods to ocean warming and acidification. Sci Rep. 9(1):4748. doi: 10.1038/s41598-019-41213-1.

                Johnson, M. et al. (2019). Influence of Green Leafy Vegetables in Diets with an Elevated ω-6:ω-3 Fatty Acid Ratio on Rat Blood Pressure, Plasma Lipids, Antioxidant Status and Markers of Inflammation. Nutrients. 11(2). pii: E301. doi: 10.3390/nu11020301.

                Statland, J.M. et al. (2019). Rasagiline for amyotrophic lateral sclerosis: A randomized, controlled trial. Muscle Nerve. 59(2):201-207. doi: 10.1002/mus.26335.

                kerstrm, B. et al. (2019).  rA1M-035, a Physicochemically Improved Human Recombinant α1-Microglobulin, Has Therapeutic Effects in Rhabdomyolysis-Induced Acute Kidney Injury. Antioxid Redox Signal. 30(4):489-504. doi: 10.1089/ars.2017.7181.

                Bednarek, N. et al. (2018). El Nio-Related Thermal Stress Coupled With Upwelling-Related Ocean Acidification Negatively Impacts Cellular to Population-Level Responses in Pteropods Along the California Current System With Implications for Increased Bioenergetic Costs. Frontiers in Marine Science. 5:1-17. doi:10.3389/fmars.2018.00486.

                Watanabe, S. et al. (2018). Detection of betacyanin in red-tube spinach (Spinacia oleracea) and its biofortification by strategic hydroponics. PLoS One. 13(9):e0203656. doi: 10.1371/journal.pone.0203656.

                Hinman, A. et al. (2018). Vitamin E hydroquinone is an endogenous regulator of ferroptosis via redox control of 15-lipoxygenase. PLoS One. 13(8):e0201369. doi: 10.1371/journal.pone.0201369.

                Gregory Rivera, M. et al. (2018). Peptide Inhibitor of Complement C1 (PIC1) demonstrates antioxidant activity via single electron transport (SET) and hydrogen atom transfer (HAT). PLoS One. 13(3):e0193931. doi: 10.1371/journal.pone.0193931.

                Ferrare, K. et al. (2018). Increase in insulin sensitivity by the association of chicoric acid and chlorogenic acid contained in a natural chicoric acid extract (NCRAE) of chicory ( Cichorium intybus L.) for an antidiabetic effect. Journal of Ethnopharmacology. 215:241–248. doi:10.1016/j.jep.2017.12.035.

                Lee, J.K. et al. (2018). Alleviation of ascorbic acid-induced gastric high acidity by calcium ascorbate in vitro and in vivo. Korean J Physiol Pharmacol. 22(1):35-42. doi: 10.4196/kjpp.2018.22.1.35.

                Sheng Cheng, H. (2017). The Ameliorative Effects of a Tocotrienol-Rich Fraction on the AGE-RAGE Axis and Hypertension in High-Fat-Diet-Fed Rats with Metabolic Syndrome. Nutrients. 9(9): 984. doi: 10.3390/nu9090984.

                Alvarenga, I.C. et al. (2017). 239 Effect of diets containing sorghum fractions on antioxidant capacity of dogs and phenolic acids of both food and plasma measured by high performance liquid chromatography (HPLC). J. Anim. Sci. 95(Suppl4):117-1.

                Smith, S.C. (2017). 237 Development of a preference ranking test with dogs. J. Anim. Sci. 95(Suppl4):116-117. doi: 10.2527/asasann.2017.237.

                ................................................................................................

                看到這兒,您心動了嗎?馬上聯系小艾吧!

                • 高品質保障 先進的生產研發技術
                • 高性價比 價宜質優,性價比高
                • 100%保證 從購買到使用,放心無憂
                • 安全運輸 完善的保護措施安全運輸

                微信掃碼在線客服

                微信咨詢

                全國免費技術支持

                400-6800-868

                在線客服
                久久精品99视频,黄鳝门视频在线播放,乳色吐息在线免费观看,欧美性爱a级大片之黑白配,717电影网good理论片
                <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <文本链> <文本链> <文本链> <文本链> <文本链> <文本链>