His標簽,也稱為多組氨酸標簽(polyhistidine),His6標簽或hexa組氨酸標簽,這是一種在轉染的細胞中目標蛋白的C端或N端上由至少6個組氨酸殘基鏈接上所組成的氨基酸序列。
His-Tag是目前用于純化的融合標簽中應用最廣泛的特點:
a:標簽較短可置于蛋白的N端或者C端
b:對蛋白的功能及下游應用影響小
c:免疫原性低,可以和多種標簽連用
d:純化方式非常簡單方便
這些屬性使His標簽成為重組蛋白生產和分離的流行標簽。然而,對于未純化的樣本,His標簽蛋白的準確定量樣品仍然很困難。
作為專業的生命科學醫藥原料供應商,Cell Biolabs中國區金牌代理,艾美捷科技為您推薦:His-Tag 蛋白ELISA 試劑盒,這是一種基于競爭性ELISA測定法,專為快速檢測和定量His標簽蛋白樣品(C 端或 N 端)而開發。
貨號 | AKR-130 |
名稱 | His-Tag Protein ELISA Kit His標簽蛋白ELSIA檢測試劑盒 |
目的 | His標簽蛋白進行定量檢測 |
原理 | 將含有His標簽的樣品或含有His標簽的重組蛋白標準品添加到已經包被好多組氨酸的微孔板上。短暫孵育后,加入抗 6xHis單克隆抗體,然后加入HRP偶聯的二抗。未知樣品中的His標簽蛋白含量是通過與由預先確定的His標簽蛋白標準品制備的標準曲線進行比較來確定的。 |
結果 | His標簽蛋白標準曲線: |
說明書 | 下載 |
引用文獻:
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